how to use graphpad prism for real time pcr

ovale mono-infections were 3.6% (18/499) and 1.0% (5/499), respectively, with the remaining being co-infections with Plasmodium falciparum. ovale among the combined study population were 18.6% (104/560) and 5.5% (31/560), respectively. More important, the cooperative primer-based assays were evaluated in a cross-sectional study using 560 samples obtained from two health facilities in Ghana. ovale cooperative primer-based assays had at least 10-fold lower detection limit compared with the corresponding conventional primer-based assays. ovale using cooperative primers that significantly limit the formation and propagation of primers-dimers. In this study, SYBR Green–based real-time PCR assays were developed for the detection of P. However, the absence of reliable Plasmodium species-specific detection tools has hampered accurate diagnosis of these minor Plasmodium species. Plasmodium malariae and Plasmodium ovale are increasingly gaining public health attention as the global transmission of falciparum malaria is decreasing.

How to use graphpad prism for real time pcr